Saline:Article Title: Intravital multiphoton imaging of mouse tibialis anterior muscle
Article Snippet: .. Reagents required: Experimental mice PBS Saline Ketamine Xylazine Evans Blue (or other vessel-labeling agent) Chemoattractant (if required) Water Equipment required: Insulin syringe with needle (BD, cat. no. 328838) Transfer pipettes (Biologix Research Company, cat. no. 30–0135) 3M paper masking tape (3M, cat. no. 2214) Deckglaser microscope cover glass (22 mm × 32 mm; Superior Marienfeld Laboratory Glassware, Paul Marienfeld, cat. no. 0101112) Medical cotton-tipped applicators (Puritan Medical Products Company, cat. no. 806-WC) Surgical microscope (Leica, model M651 MSD) Custom-built muscle-imaging stage platform (see EQUIPMENT SETUP for details) McPherson-Vannas Micro Dissecting Spring Scissors (Roboz, cat. no. RS-5600) Dumont Tweezers (Roboz, cat. no. RS-5047) Extra Fine Micro Dissecting Scissors (Roboz, cat. no. RS-5880) Curved splinter forceps (Aesculap, B. Braun Melsungen, cat. no. BD314R) Braun cruZer6 precision trimmer (Braun) 1 mL Luer Slip Tuberculin Syringe (BD, cat. no. 302100) Re-usable adhesive (Blu-tack) Polyethylene tubing for catheter (Microtube Extrusions, cat. no. PE6128) BD PrecisionGlide 30G × 1/2 in. needle (BD, cat. no. 305106) Black braided silk surgical suture (Roboz, cat. no. SUT-15–1) C-fold towels (Kimberly-Clark, cat. no. 20311) Dow Corning ® high vacuum grease 18.5 × 20.5 cm plastic board Equipment setup: Magnets (4.0 × 2.0 × 1.5 cm) Metal base plate (19.5 × 19.5 cm) Styrofoam sponge (12.5 × 10.0 cm or cut to the size of available heating pad) Heating pad (Fine Science Tools, cat. no. 21060–00) Curved holder, for positioning the control thermistor Interface cable CC-28 with DIN connector and thermistors, one for the (temperature control and the other for the temperature monitor ((Warner Instruments (Harvard Apparatus), cat. no. 640106) Resistive heater block RH-2 (Warner Instruments (Harvard Apparatus), (cat. no. 640274) ( Temperature controller TC-344B for the ear platform (Warner Instruments ((Harvard Apparatus), cat. no. 640101) Temperature controller TR-200 for mouse heating pad (Fine Science Tools, (cat. no. 21052–00) ( Power supply for TR-200 (Fine Science Tools, cat. no. 21051–00) Animal rectal probe (Fine Science Tools, cat. no. 21060–01) C-fold towels (Kimberly-Clark, cat. no. 20311) Coverslip support holder: Plastic base (5.0 × 3.5 × 0.5 cm) with magnet embedded. ..
Microscopy:Article Title: Intravital multiphoton imaging of mouse tibialis anterior muscle
Article Snippet: .. Reagents required: Experimental mice PBS Saline Ketamine Xylazine Evans Blue (or other vessel-labeling agent) Chemoattractant (if required) Water Equipment required: Insulin syringe with needle (BD, cat. no. 328838) Transfer pipettes (Biologix Research Company, cat. no. 30–0135) 3M paper masking tape (3M, cat. no. 2214) Deckglaser microscope cover glass (22 mm × 32 mm; Superior Marienfeld Laboratory Glassware, Paul Marienfeld, cat. no. 0101112) Medical cotton-tipped applicators (Puritan Medical Products Company, cat. no. 806-WC) Surgical microscope (Leica, model M651 MSD) Custom-built muscle-imaging stage platform (see EQUIPMENT SETUP for details) McPherson-Vannas Micro Dissecting Spring Scissors (Roboz, cat. no. RS-5600) Dumont Tweezers (Roboz, cat. no. RS-5047) Extra Fine Micro Dissecting Scissors (Roboz, cat. no. RS-5880) Curved splinter forceps (Aesculap, B. Braun Melsungen, cat. no. BD314R) Braun cruZer6 precision trimmer (Braun) 1 mL Luer Slip Tuberculin Syringe (BD, cat. no. 302100) Re-usable adhesive (Blu-tack) Polyethylene tubing for catheter (Microtube Extrusions, cat. no. PE6128) BD PrecisionGlide 30G × 1/2 in. needle (BD, cat. no. 305106) Black braided silk surgical suture (Roboz, cat. no. SUT-15–1) C-fold towels (Kimberly-Clark, cat. no. 20311) Dow Corning ® high vacuum grease 18.5 × 20.5 cm plastic board Equipment setup: Magnets (4.0 × 2.0 × 1.5 cm) Metal base plate (19.5 × 19.5 cm) Styrofoam sponge (12.5 × 10.0 cm or cut to the size of available heating pad) Heating pad (Fine Science Tools, cat. no. 21060–00) Curved holder, for positioning the control thermistor Interface cable CC-28 with DIN connector and thermistors, one for the (temperature control and the other for the temperature monitor ((Warner Instruments (Harvard Apparatus), cat. no. 640106) Resistive heater block RH-2 (Warner Instruments (Harvard Apparatus), (cat. no. 640274) ( Temperature controller TC-344B for the ear platform (Warner Instruments ((Harvard Apparatus), cat. no. 640101) Temperature controller TR-200 for mouse heating pad (Fine Science Tools, (cat. no. 21052–00) ( Power supply for TR-200 (Fine Science Tools, cat. no. 21051–00) Animal rectal probe (Fine Science Tools, cat. no. 21060–01) C-fold towels (Kimberly-Clark, cat. no. 20311) Coverslip support holder: Plastic base (5.0 × 3.5 × 0.5 cm) with magnet embedded. ..
Adhesive:Article Title: Intravital multiphoton imaging of mouse tibialis anterior muscle
Article Snippet: .. Reagents required: Experimental mice PBS Saline Ketamine Xylazine Evans Blue (or other vessel-labeling agent) Chemoattractant (if required) Water Equipment required: Insulin syringe with needle (BD, cat. no. 328838) Transfer pipettes (Biologix Research Company, cat. no. 30–0135) 3M paper masking tape (3M, cat. no. 2214) Deckglaser microscope cover glass (22 mm × 32 mm; Superior Marienfeld Laboratory Glassware, Paul Marienfeld, cat. no. 0101112) Medical cotton-tipped applicators (Puritan Medical Products Company, cat. no. 806-WC) Surgical microscope (Leica, model M651 MSD) Custom-built muscle-imaging stage platform (see EQUIPMENT SETUP for details) McPherson-Vannas Micro Dissecting Spring Scissors (Roboz, cat. no. RS-5600) Dumont Tweezers (Roboz, cat. no. RS-5047) Extra Fine Micro Dissecting Scissors (Roboz, cat. no. RS-5880) Curved splinter forceps (Aesculap, B. Braun Melsungen, cat. no. BD314R) Braun cruZer6 precision trimmer (Braun) 1 mL Luer Slip Tuberculin Syringe (BD, cat. no. 302100) Re-usable adhesive (Blu-tack) Polyethylene tubing for catheter (Microtube Extrusions, cat. no. PE6128) BD PrecisionGlide 30G × 1/2 in. needle (BD, cat. no. 305106) Black braided silk surgical suture (Roboz, cat. no. SUT-15–1) C-fold towels (Kimberly-Clark, cat. no. 20311) Dow Corning ® high vacuum grease 18.5 × 20.5 cm plastic board Equipment setup: Magnets (4.0 × 2.0 × 1.5 cm) Metal base plate (19.5 × 19.5 cm) Styrofoam sponge (12.5 × 10.0 cm or cut to the size of available heating pad) Heating pad (Fine Science Tools, cat. no. 21060–00) Curved holder, for positioning the control thermistor Interface cable CC-28 with DIN connector and thermistors, one for the (temperature control and the other for the temperature monitor ((Warner Instruments (Harvard Apparatus), cat. no. 640106) Resistive heater block RH-2 (Warner Instruments (Harvard Apparatus), (cat. no. 640274) ( Temperature controller TC-344B for the ear platform (Warner Instruments ((Harvard Apparatus), cat. no. 640101) Temperature controller TR-200 for mouse heating pad (Fine Science Tools, (cat. no. 21052–00) ( Power supply for TR-200 (Fine Science Tools, cat. no. 21051–00) Animal rectal probe (Fine Science Tools, cat. no. 21060–01) C-fold towels (Kimberly-Clark, cat. no. 20311) Coverslip support holder: Plastic base (5.0 × 3.5 × 0.5 cm) with magnet embedded. ..
Control:Article Title: Intravital multiphoton imaging of mouse tibialis anterior muscle
Article Snippet: .. Reagents required: Experimental mice PBS Saline Ketamine Xylazine Evans Blue (or other vessel-labeling agent) Chemoattractant (if required) Water Equipment required: Insulin syringe with needle (BD, cat. no. 328838) Transfer pipettes (Biologix Research Company, cat. no. 30–0135) 3M paper masking tape (3M, cat. no. 2214) Deckglaser microscope cover glass (22 mm × 32 mm; Superior Marienfeld Laboratory Glassware, Paul Marienfeld, cat. no. 0101112) Medical cotton-tipped applicators (Puritan Medical Products Company, cat. no. 806-WC) Surgical microscope (Leica, model M651 MSD) Custom-built muscle-imaging stage platform (see EQUIPMENT SETUP for details) McPherson-Vannas Micro Dissecting Spring Scissors (Roboz, cat. no. RS-5600) Dumont Tweezers (Roboz, cat. no. RS-5047) Extra Fine Micro Dissecting Scissors (Roboz, cat. no. RS-5880) Curved splinter forceps (Aesculap, B. Braun Melsungen, cat. no. BD314R) Braun cruZer6 precision trimmer (Braun) 1 mL Luer Slip Tuberculin Syringe (BD, cat. no. 302100) Re-usable adhesive (Blu-tack) Polyethylene tubing for catheter (Microtube Extrusions, cat. no. PE6128) BD PrecisionGlide 30G × 1/2 in. needle (BD, cat. no. 305106) Black braided silk surgical suture (Roboz, cat. no. SUT-15–1) C-fold towels (Kimberly-Clark, cat. no. 20311) Dow Corning ® high vacuum grease 18.5 × 20.5 cm plastic board Equipment setup: Magnets (4.0 × 2.0 × 1.5 cm) Metal base plate (19.5 × 19.5 cm) Styrofoam sponge (12.5 × 10.0 cm or cut to the size of available heating pad) Heating pad (Fine Science Tools, cat. no. 21060–00) Curved holder, for positioning the control thermistor Interface cable CC-28 with DIN connector and thermistors, one for the (temperature control and the other for the temperature monitor ((Warner Instruments (Harvard Apparatus), cat. no. 640106) Resistive heater block RH-2 (Warner Instruments (Harvard Apparatus), (cat. no. 640274) ( Temperature controller TC-344B for the ear platform (Warner Instruments ((Harvard Apparatus), cat. no. 640101) Temperature controller TR-200 for mouse heating pad (Fine Science Tools, (cat. no. 21052–00) ( Power supply for TR-200 (Fine Science Tools, cat. no. 21051–00) Animal rectal probe (Fine Science Tools, cat. no. 21060–01) C-fold towels (Kimberly-Clark, cat. no. 20311) Coverslip support holder: Plastic base (5.0 × 3.5 × 0.5 cm) with magnet embedded. ..
Blocking Assay:Article Title: Intravital multiphoton imaging of mouse tibialis anterior muscle
Article Snippet: .. Reagents required: Experimental mice PBS Saline Ketamine Xylazine Evans Blue (or other vessel-labeling agent) Chemoattractant (if required) Water Equipment required: Insulin syringe with needle (BD, cat. no. 328838) Transfer pipettes (Biologix Research Company, cat. no. 30–0135) 3M paper masking tape (3M, cat. no. 2214) Deckglaser microscope cover glass (22 mm × 32 mm; Superior Marienfeld Laboratory Glassware, Paul Marienfeld, cat. no. 0101112) Medical cotton-tipped applicators (Puritan Medical Products Company, cat. no. 806-WC) Surgical microscope (Leica, model M651 MSD) Custom-built muscle-imaging stage platform (see EQUIPMENT SETUP for details) McPherson-Vannas Micro Dissecting Spring Scissors (Roboz, cat. no. RS-5600) Dumont Tweezers (Roboz, cat. no. RS-5047) Extra Fine Micro Dissecting Scissors (Roboz, cat. no. RS-5880) Curved splinter forceps (Aesculap, B. Braun Melsungen, cat. no. BD314R) Braun cruZer6 precision trimmer (Braun) 1 mL Luer Slip Tuberculin Syringe (BD, cat. no. 302100) Re-usable adhesive (Blu-tack) Polyethylene tubing for catheter (Microtube Extrusions, cat. no. PE6128) BD PrecisionGlide 30G × 1/2 in. needle (BD, cat. no. 305106) Black braided silk surgical suture (Roboz, cat. no. SUT-15–1) C-fold towels (Kimberly-Clark, cat. no. 20311) Dow Corning ® high vacuum grease 18.5 × 20.5 cm plastic board Equipment setup: Magnets (4.0 × 2.0 × 1.5 cm) Metal base plate (19.5 × 19.5 cm) Styrofoam sponge (12.5 × 10.0 cm or cut to the size of available heating pad) Heating pad (Fine Science Tools, cat. no. 21060–00) Curved holder, for positioning the control thermistor Interface cable CC-28 with DIN connector and thermistors, one for the (temperature control and the other for the temperature monitor ((Warner Instruments (Harvard Apparatus), cat. no. 640106) Resistive heater block RH-2 (Warner Instruments (Harvard Apparatus), (cat. no. 640274) ( Temperature controller TC-344B for the ear platform (Warner Instruments ((Harvard Apparatus), cat. no. 640101) Temperature controller TR-200 for mouse heating pad (Fine Science Tools, (cat. no. 21052–00) ( Power supply for TR-200 (Fine Science Tools, cat. no. 21051–00) Animal rectal probe (Fine Science Tools, cat. no. 21060–01) C-fold towels (Kimberly-Clark, cat. no. 20311) Coverslip support holder: Plastic base (5.0 × 3.5 × 0.5 cm) with magnet embedded. ..
Dissection:Article Title: Primary Embryonic Rat Cortical Neuronal Culture and Chronic Rotenone Treatment in Microfluidic Culture Devices
Article Snippet: Motic Dissection microscope (Motic, model: SMZ-168) 5. .. Roboz surgical instrument dissection tools a. Dumont #5 Forceps, Straight tip (Roboz, catalog number: RS-5060) b. Dumont #5 Forceps, 45 Degree tip (Roboz, catalog number: RS-5005) c. Straight, Sharp-Blunt Operating Scissors (Roboz, catalog number: RS-6812) d. Extra Fine Micro Dissecting Scissors (Roboz, catalog number: RS-5882) e. Micro Dissecting Spring Scissors (Roboz, catalog number: RS-5603) 6. .. Sorvall Legend T table-top centrifuge (Thermo, model: 75004367) 7.
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